Day 3

Dr. P added bacteriophage virus T4 to kill the bacteria from one group's unknown sample agar petri dish. In the shape of JMJ and a "dot."

Results from Environmental Sample

• A few small white dot colonies, circular, raised

Day 2

Unknown sample 5
Results after incubating at 37 degrees overnight
Agar Petri Dish

• Successful bacterial growth, off white/creme colored bacterial growth

Slant agar test tube

• Successful bacterial growth, off white bacteria

Broth

• Clouded broth, very small creme colored particles floating

Placing the agar plate under a high tech (find the name out of) Microscope to see closer morphology

Agar Petri Dish

• Circular, creme colored, raised, undulate

Environmental Sample

Swabbed Cara Egan's cast on her hand then transferred to a agar petri dish and incubated overnight at 37 degrees (CHECK ON TEMPERATURE) .

Day 1

Each lab group was given an unknown sample of bacteria to perform various tests on to determine its identity. We were given sample #5. From the original stock of sample we, by means of aseptic transfer, transferred the bacteria to an Agar Plate, Agar Slant test tube and nutrient broth to culture new bacteria.

Original Stock for Sample #5

Aseptic Technique

 Depicted below are the ingredients used to prepare each of the three environments for culturing bacteria. (Nutrient Agar and Mannitol Salt Agar are both used to prepare agar plates/slants however certain bacteria may not grow in the Mannitol Salt Agar due to their nutritional needs.) From the original

Ingredient to Make Agar Plate

Incubated at 37 degrees

Ingredient for Nutrient Broth

Finger Print Agar Plate

Ingredient to Make Agar Plate

Preparing a Streak Plate

Preparing an Agar Slant Test Tube